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Density gradient centrifugation of UCB Separation into Plasma-PBS phase, PBMC phase, Pancoll phase and RBC (Erythrocytes) phase. PBMC: peripheral blood mononuclear cells.

Journal: STAR Protocols

Article Title: Protocol for CRISPR-Cas9-mediated induction of KMT2A rearrangements in cell line and umbilical cord blood hematopoietic stem and progenitor cells

doi: 10.1016/j.xpro.2024.103481

Figure Lengend Snippet: Density gradient centrifugation of UCB Separation into Plasma-PBS phase, PBMC phase, Pancoll phase and RBC (Erythrocytes) phase. PBMC: peripheral blood mononuclear cells.

Article Snippet: Pancoll human (1.077 g/mL) density gradient , PAN-Biotech , Cat# P04-60500.

Techniques: Gradient Centrifugation

Journal: STAR Protocols

Article Title: Protocol for CRISPR-Cas9-mediated induction of KMT2A rearrangements in cell line and umbilical cord blood hematopoietic stem and progenitor cells

doi: 10.1016/j.xpro.2024.103481

Figure Lengend Snippet:

Article Snippet: Pancoll human (1.077 g/mL) density gradient , PAN-Biotech , Cat# P04-60500.

Techniques: Cell Culture, Recombinant, Red Blood Cell Lysis, Reverse Transcription, TA Cloning, Software

List of reagents with supplier details.

Journal: Cells

Article Title: Evidence for a Role of the Long Non-Coding RNA ITGB2-AS1 in Eosinophil Differentiation and Functions

doi: 10.3390/cells13231936

Figure Lengend Snippet: List of reagents with supplier details.

Article Snippet: Briefly, WBCs were separated by density-gradient centrifugation (800× g , 20 min, room temperature (RT)) using Pancoll Human (density of 1.077 g/mL) (PAN-Biotech).

Techniques: Staining, Saline, Isolation, Western Blot, SYBR Green Assay, Plasmid Preparation, Electron Microscopy, Bicinchoninic Acid Protein Assay, Protease Inhibitor, Transfection